The rate of reaction of Succinate dehydrogenase :: GCSE Chemistry Coursework Investigation

The say of reaction of Succinate dehydrogenaseIntroductionEnzymes ar protein molecules that function as biological catalyststhat can armed service break larger molecules into smaller molecules whileremaining unchanged themselves. They speed up the chemical reactionsby lowering the energy of activation barrier, are specific to onemolecule. The enzymes specificity arises from its active site, anarea with a shape corresponding to the molecule with which it reacts(the substrate). The shape of the enzyme where the chemical binds onlyallows the binding of that particular chemical, or inhibitorsubstrates that are structually similar to the substrate, competingfor the active site. The enzyme and the substrate slot together (likea key for a lock, or by induced fit) forming an enzymesubstratecomplex that allows the reaction to experience place.An enzymes activity is affected by its environment. Each enzyme has atemperature and pH level at which its activity is greatest and thereaction it catalyses proceeds at its fastest rate. The rate ofenzyme-catalysed reactions increases as the temperature and pHbalances approach its optimum level. At higher or lower temperaturesand pH balances, the enzyme molecules become damaged or denatured.They cannot catalyse the reaction very(prenominal) well, if at all, and the damageis usually permanent (Campbell, et al, 2006).The aim of this study was to investigate the rate of reaction ofsuccinate dehydrogenase, an enzyme extracted from chicken hearts. Therate of reaction was analysed considering two factors pH andtemperature. The baron for the enzyme succinate dehydrogenase tooxidise two alternative substrates (malonate and propionate) will alsobe examined.Materials and MethodPart 1. Effect of pH on enzyme activityBlender50 grams of modern chicken hearts - 2 days old purchased from localbutcher Rays meats Sorrento. Chicken hearts were kept in fridge untilprepared the evening of purchase.3 test tubesDistilled water0.2M Na2HPO4 with 1 00ml distilled water ( settlement 1)0.2M NaH2PO4 with 100ml distilled water (solution 2)0.1M succinate with 100ml distilled water0.0003 M DPIP with 100ml distilled waterBUFFERSpH 5 buffer 1ml solution 1 to 49ml of solution 2, mix fit 50 mldistilled waterpH 7.3 buffer 75ml solution 1 to 25ml solution 2, mix add 100mldistilled waterpH 9 buffer - 10ml solution 1 to 10ml distilled waterStopwatchEnzyme preparation (Wright, 2005)Chicken hearts were prepared according to notes in Lab (Wright 2005).This liquid formed the enzyme.A rack containing 3 test tubes were arranged containingtube 1 5.8 ml pH5 buffer1 ml 0.1M succinate.2 ml enzymetube 2. - 5.8 ml pH7.3 buffer1 ml 0.1M succinate.2 ml enzymetube 3. - 5.8 ml pH9 buffer1 ml 0.